PDPK1 (N-term) Peptide
产品名称： PDPK1 (N-term) Peptide
英文名称： PDPK1 (N-term) Peptide
产品编号： P2133
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
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- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of PDPK1.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PDPK1.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Peroxisomal targeting as a tool for assaying potein-protein interactions in the living cell: cytokine-independent survival kinase (CISK) binds PDK-1 in vivo in a phosphorylation-dependent manner.
Nilsen T, Slagsvold T, Skjerpen CS, Brech A, Stenmark H, Olsnes S.J Biol Chem. 2004 Feb 6;279(6):4794-801. Epub 2003 Nov 6.
- 2.
- In vivo role of the PIF-binding docking site of PDK1 defined by knock-in mutation.
Collins BJ, Deak M, Arthur JS, Armit LJ, Alessi DR.EMBO J. 2003 Aug 15;22(16):4202-11.
- 3.
- Membrane localization of 3-phosphoinositide-dependent protein kinase-1 stimulates activities of Akt and atypical protein kinase C but does not stimulate glucose transport and glycogen synthesis in 3T3-L1 adipocytes.
Egawa K, Maegawa H, Shi K, Nakamura T, Obata T, Yoshizaki T, Morino K, Shimizu S, Nishio Y, Suzuki E, Kashiwagi A.J Biol Chem. 2002 Oct 11;277(41):38863-9. Epub 2002 Jul 29.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of PDPK1 polyclonal antibody (Cat # PAB4567) in HL-60 cell lysate . PDPK1 (arrow) was detected using purified PDPK1 polyclonal antibody (Cat # PAB4567) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PDPK1 polyclonal antibody (Cat # PAB4567) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .
- Interactome
- Related Disease