PRKCD monoclonal antibody (M02), clone 6A2
产品名称： PRKCD monoclonal antibody (M02), clone 6A2
英文名称： PRKCD monoclonal antibody (M02), clone 6A2
产品编号： H00005580-M02
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant PRKCD.
- Immunogen:
- PRKCD (NP_006245, 577 a.a. ~ 676 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- DILEKLFEREPTKRLGVTGNIKIHPFFKTINWTLLEKRRLEPPFRPKVKSPRDYSNFDQEFLNEKARLSYSDKNLIDSMDQSAFAGFSFVNPKFEHLLED
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG2a Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
- MSDS:
Download
- Applications
- Western Blot (Transfected lysate)
- Western Blot analysis of PRKCD expression in transfected 293T cell line by PRKCD monoclonal antibody (M02), clone 6A2.
Lane 1: PRKCD transfected lysate(77.5 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged PRKCD is approximately 0.1ng/ml as a capture antibody.
- Protocol Download
- RNAi Knockdown (Antibody validated)
- Western blot analysis of PRKCD over-expressed 293 cell line, cotransfected with PRKCD Validated Chimera RNAi ( Cat # H00005580-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PRKCD monoclonal antibody (M02) clone 6A2 (Cat # H00005580-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Protocol Download
- Entrez GeneID:
- 5580
- GeneBank Accession#:
- NM_006254
- Protein Accession#:
- NP_006245
- Gene Name:
- PRKCD
- Gene Alias:
- MAY1,MGC49908,PKCD,nPKC-delta
- Gene Description:
- protein kinase C, delta
- Omim ID:
- 176977
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq
- Other Designations:
- protein kinase C delta VIII