CD20,CD20抗体

产品编号HZ-20639R
英文名称CD20
中文名称CD20抗体
别 名B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; MS4A1; CD20; CD20_HUMAN.
说 明 书0.1ml 0.2ml
研究领域肿瘤 细胞生物 免疫学 干细胞 淋巴细胞 b-淋巴细胞
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat,
CD20,CD20抗体产品应用WB=1:100-500 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:30-300 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量33kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human CD20
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
CD20,CD20抗体PubMedPubMed
产品介绍background:
CD20 is a non glycosylated protein with a molecular weight of 35 or 37 kDa depending on the degree of phosphorylation. Although not a member of the tetraspanin superfamily of cell surface receptors, it crosses the cell membrane four times. The CD20 antigen is present on human pre B lymphocytes and on B lymphocytes at all stages of maturation, except on plasma cells. Low level expression of the CD20 antigen has been detected on normal T lymphocytes. The CD20 molecule is involved in regulation of B cell differentiation, presumably via its reported function as a Ca++ channel subunit.

Function:
This protein may be involved in the regulation of B-cell activation and proliferation

Subcellular Location:
Membrane; Multi-pass membrane protein.

Tissue Specificity:
Expressed on B-cells.

Post-translational modifications:
Phosphorylated. Might be functionally regulated by protein kinase(s).

DISEASE:
Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low.

Similarity:
Belongs to the MS4A family.

Gene ID:
931

Database links:
Entrez Gene: 931 Human
Omim: 112210 Human
SwissProt: P11836 Human
Unigene: 712553 Human

CD20,CD20抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

B淋巴细胞标志物

CD20是B细胞特异性抗原，其表达呈细胞类型一种质膜蛋白质，参与信号传导作用，此抗体识别大多数B细胞上的一种抗原。多用于标记B淋巴细胞及B细胞淋巴瘤。
CD20一般不与T细胞交叉反应，对B细胞淋巴瘤和急慢性淋巴细胞白血病等有较好的细胞特异性，因此在恶性淋巴瘤，尤其是T或B细胞淋巴瘤的分类上，CD20是最常用的B细胞标志。