SuperSignal West Pico Chemiluminescent Substrate

Thermo Scientific SuperSignal West Pico Chemiluminescent Substrateisan enhanced chemiluminescent (ECL) HRP substrate for low-picogram-level detection by Western blot analysis.

SuperSignal West Pico Substrate emits light for longer and chemiluminescent signal at more than twice the intensity of other entry-level luminol-based horseradish peroxidase detection systems while also maintaining sensitivity and low background. In addition, the greater signal intensity is achieved using less primary and secondary antibody than typical ECL substrates. All of this for about 30% less cost than comparable products.

Highlights:

• ECL substrate– an enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP) activity from antibodies and other Western blot probes
• Economy– costs less per milliliter than other similarly sensitive chemiluminescent substrates
• Picogram sensitivity– highly sensitive for the rapid development of a wide range of protein levels
• Stable light output– long-duration signal makes it possible to make multiple exposures to film
• High intensity– signal is twice as strong as output from other luminol-based systems
• Excellent stability– 24-hour working solution stability; 1-year kit stability at room temperature
• Saves antibody– requires much less (more dilute) primary and secondary antibodies per blot

Product Details:

SuperSignal West Pico Chemiluminescent Substrate is stable for at least one year at room temperature and can be stored at 4°C for longer stability. The working solution is stable for a minimum of 24 hours at room temperature and can be used in either light or dark conditions.

In side-by-side comparisons using identical conditions, blots incubated in SuperSignal West Pico Chemiluminescent Substrate exhibit at least twice the intensity of blots treated with the GE Healthcare ECL System. SuperSignal West Pico Chemiluminescent Substrate costs much less than the GE Healthcare ECL System. Furthermore, the SuperSignal West Pico Substrate stock solution is room temperature (RT) stable for more than 12 months, with no discernable loss in activity. RT stability frees up valuable cold room space and saves time because there is no need to wait for the reagents to warm up. With signal duration of more than six hours, there is adequate time to optimize the exposure conditions. In most cases, there is no need to rerun gels.Switching to SuperSignal West Pico Substrate from an entry-level ECL substrate is not only easy, it will give you more of the results you are looking for.

Enhance light emission kinetics: Thermo Scientific SuperSignal Substrate vs. GE ECL System.Net relative intensity six hours after incubation is much greater for SuperSignal West Pico Substrate than for the GE ECL System.

Thermo Scientific SuperSignal West Pico is more sensitive than GE ECL Substrate.Recombinant mouse IL-2 was diluted (50 to 0.003ng) and electrophoresis was performed. The gels were transferred to nitrocellulose membranes, blocked and incubated with a 1µg/mL dilution of rat anti-mouse IL-2. After washing, the membranes were incubated with 20ng/mL dilutions of HRP-conjugated goat anti-rat antibody. The membranes were washed again and then incubated with substrate that was prepared according to the manufacturers instructions. Blots were exposed to film for one- and five-minute exposures