分子量:45 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human PLAU, His Tag by the LAL method.

Buffer:50 mM NaAc, 100 mM NaCl, pH5.0

生物活性:Measured by its ability to bind human PLAUR in a function ELISA.

产品特性:Human PLAU, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 45 kDa. The predicted N-terminus is Ser21,Ile179&Lys156. The reducing (R) protein migrates as three bands corresponding to the long α chain, β chain and unprocessed full-length chain with the molecular mass of 18 kDa, 32 kDa and 50 kDa respectively in SDS-PAGE  due to glycosylation and cleavage.

产品背景:Urokinase - type plasminogen activator is also known as PLAU and UPA, a serine protease with an extremely limited substrate specificity, cleaving the sequence Cys  Pro  Gly - Arg560 - Val561  Val  Gly  Gly  Cys in plasminogen to form plasmin. uPA is a potent marker of invasion and metastasis in a variety of human cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium.uPA and its receptor (uPAR) have been implicated in a broad spectrum of pathophysiological processes, including fibrinolysis, proteolysis, inflammation, atherogenesis and plaque destabilization, all of which are involved in the pathogenesis of MI (myocardial infarction).