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上海信裕生物科技有限公司
RB1抗体

RB1抗体

商家询价

产品名称： RB1抗体

英文名称： RB1Antibody

产品编号： XY10048-2

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

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 RB1抗体Applications

In-house applications ELISA, WB, IHC, IP     Application not tested? Please see our Guarantee.
Published applications IHC, WB Publications 15 citations –view here
Species specificity Human, Mouse; other species not tested.
Published Species Human, mouse
Images
Prev1NextView more validation data
  • 10048-2-Ig;Jurkat cellsJurkat cells were subjected to SDS PAGE followed by western blot with 10048-2-Ig(RB1 antibody) at dilution of 1:3000
  • 10048-2-Ig;A431 cellsIP Result of anti-RB1 (IP:10048-2-Ig, 3ug; Detection:10048-2-Ig 1:500) with A431 cells lysate 3000ug.
  • 10048-2-Ig;human prostate cancerImmunohistochemical of paraffin-embedded human prostate cancer using 10048-2-Ig(RB1 antibody) at dilution of 1:200 (under 10x lens)
Positive WB detected in Jurkat cells, A431 cells
Positive IP detected in A431 cells
Positive IHC detected in Human prostate cancer tissue, human lung cancer tissue
Recommended dilution WB : 1:1000-1:10000
IP : 1:200-1:2000
IHC : 1:50-1:200
 RB1抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Protein A purification
Isotype IgG Storage PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information RB1抗体
Immunogen RB1 fusion protein ag0006 Full name Retinoblastoma 1
Calculated molecular
weight		 110 kDa Observed molecular 
weight		 110 kDa
GenBank accession 
number		 BC040540 Gene ID (NCBI) 5925
Gene symbol RB1 Synonyms OSRC, p105 Rb, pp110, pRb, RB, RB1, retinoblastoma 1
Background

RB1, also named as pp110, pRb and p105 Rb, belongs to the retinoblastoma protein (RB) family. It is a key regulator of entry into cell division that acts as a tumor suppressor. RB1 acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. It recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. RB1 controls histone H4 'Lys-20' trimethylation and inhibits the intrinsic kinase activity of TAF1. It mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. This antibody is a rabbit polyclonal antibody raised against human RB1 fusion protein. RB1抗体

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