分子量:48.4 Kda

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human IL-2 R alpha, Fc Tag by the LAL method.

Buffer:50 mM tris, 100 mM glycine, pH7.5

生物活性:

产品特性:Human IL-2 R alpha, Fc Tag is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 48.4 Kda. The predicted N-terminus is Glu 22. The reducing (R) protein migrates as 60-75 kDa in SDS-PAGE  due to glycosylation.

产品背景:Interleukin-2 receptor subunit alpha (IL2RA) is also known as IL-2R subunit alpha, IL-2-RA, IL2-RA, TAC antigen, p55, CD antigen CD25, is a type I transmembrane glycoprotein. IL2RA is expressed on activated T cells and regulatory T cells, and is capable of binding IL2 with low affinity by itself. However, a ligand-induced high affinity heterotrimeric receptor complex is produced when IL2RA is associated non-covelently with the IL2 receptor beta and gamma chain, and subsequently initiates the intacellular signal pathways such as MAPK or JAK/STAT. On dendritic cells (DC), CD25 has been previously regarded as an activation marker, while both murine and human DC can express CD25, they do not express the beta-chain of the IL-2 receptor, which is indispensable for the execution of IL-2 signaling.