分子量:31.2 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Mouse Fc gamma RI, His Tag by the LAL method.

Buffer:PBS, pH7.4

生物活性:The bioactivity is measured by its binding ability to mouse IgG2a in a SPR assay.
Immobilized Mouse Fc gamma RI / CD64 Protein, His Tag (Cat# CD4-M5227) can bind mouse IgG2a with affinity constant of around 6.1 nM.

产品特性:Mouse Fc gamma RI, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 31.2 kDa. The predicted N-terminus is Glu 25. The reducing (R) protein migrates as 40-55 kDa in SDS-PAGE  due to glycosylation.

产品背景:Receptors that recognize the Fc portion of IgG are divided into three groups designated Fc gamma RI, RII, and RIII, also known respectively as CD64, CD32, and CD16. Fc gamma RI binds IgG with high affinity and functions during early immune responses. Fc gamma RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses.
High affinity immunoglobulin gamma Fc receptor I is also known as FCGR1A, FCG1, FCGR1, CD64 and IGFR1, is a type of integral membrane glycoprotein that binds monomeric IgG-type antibodies with high affinity, which belongs to the immunoglobulin superfamily or FCGR1 family. FCGR1A / CD64 contains 3 Ig-like C2-type (immunoglobulin-like) domains. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells.