分子量:63.4 kDa

纯度:>90% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Mouse ADAM17, His Tag by the LAL method.

Buffer:50 mM MES, 150 mM NaCl, pH6.5

生物活性:

产品特性:Mouse ADAM17, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 63.4 kDa. The predicted N-terminus is Pro 18,Asp 59 and Arg 215. The reducing (R) protein migrates as 19 kDa and 20 kDa(Propeptide),55-66 kDa (Mature-ADAM17) and 75-94 kDa(Pro-ADAM17)
 in SDS-PAGE  due to glycosylation.

产品背景:Disintegrin and metalloproteinase domain-containing protein 17 (ADAM17), a member of the ADAM protein family of disintegrins and metalloproteases, is also known as TNF-alpha convertase, TNF-alpha-converting enzyme and CD156b, which contains one disintegrin domain and one peptidase M12B domain. ADAM17 can cleave the membrane-bound precursor of TNF-alpha to its mature soluble form. ADAM17 is also responsible for the proteolytical release of soluble JAM3 from endothelial cells surface (By similarity) and proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Furthermore, ADAM17 acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation.