NFκB信号通路ChIP qPCR芯片分析NFκB介导的信号通路相关的48个关键基因的组蛋白修饰状态或组蛋白密码。组蛋白修饰调节染色质结构和与相关基因的转录活性。NFκB家族是一组转录因子由压力和炎症反应激活。其他NfκB可以刺激生长因子、细胞因子,病原体相关分子产物(PAMPs)和DNA损伤等。NFκB应答基因调控过程,如细胞凋亡、组织重构、肿瘤发生和炎症。NFκB转录反应还涉及表观遗传因子,如组蛋白去乙酰酶抑制剂(hdac)，增强NFκB在靶标基因启动子上的活性。NFκB通路分析组蛋白修饰和NFκB靶标标基因，可以助于洞察调节NFκB信号通路的分子和表观遗传机制。NFκB,这个芯片包含Rel, NFκB和 IκB的家族成员, NFκB靶标基因，以及激活这个通路的细胞外配体和细胞表面受体。

Activation of NFκB Pathway:
Ligands and Receptors: IL1B, IL8, MYD88, TLR1, TLR2, TLR3, TLR4, TLR6, TLR8, TOLLIP, TNF, TNFRSF10A, TNFRSF10B.
Kinases: CHUK (IKKa), IRAK1, IRAK2.
IκB kinase/NFκB Cascade: EDARADD, IKBKB, IRAK2, STAT1, TRAF2, TLR8.
Cytoplasmic Sequestering and Release of NFκB: BCL3, IL10, NLRP12 (NALP12), NFKBIA (IkBa/MAD3), CD27 (TNFRSF7), TNFSF14.
Transcription Factors: NFKB1, NFKB2, NFKBIA, RELA, RELB, STAT1, TNF.
Inflammatory Response: IL1B, IL8, IRAK2, MYD88, NFKB1, TLR1, TLR2, TLR3, TLR4, TLR6, TLR8, TNF.

Positive Regulation of IκB Kinase and the NFκB Cascade:
Ligands and Receptors: CD40 (TNFRSF5), F2R, FASLG (TNFSF6), LTBR, SLC20A1, TICAM2 (TRAM), TNFRSF1A, TNFSF10 (TRAIL), TICAM1 (TRIF).
Membrane Molecules: GJA1, HMOX1, LPAR1 (EDG2), RHOA.
Kinases: RIPK1, TBK1.
Other: BCL10, BIRC2 (c-IAP2), NOD1 (CARD4), CASP1 (ICE), CASP8 (FLICE), CFLAR (CASPER), FADD, IKBKE, MALT1, PPM1A, REL, TRIM13 (RFP2), TRADD.

NFκB Responsive Genes:
Extracellular and Adhesion Molecules: AGT, CSF1 (MCSF), CSF2 (GM-CSF), CSF3 (GCSF), ICAM1, IFNA1, IFNG.
Cytokines: CCL2 (MCP-1), CCL5 (RANTES), CSF1 (MCSF), CSF2 (GM-CSF), CSF3 (GCSF), IFNA1, IFNG, IL8, IRF1, LTA (TNFB), TNF.
Apoptosis: BCL2A1 (Bfl-1/A1), BCL2L1 (BCL-X), BIRC3 (c-IAP1).

Other Factors Involved in the NFκB Pathway: 
Ligands and Receptors: IL1R1, RAF1, TLR7, TLR9.
Kinases: AKT1, RAF1.
Transcription Factors: ATF1, EGR1, ELK1, FOS, JUN, TNFAIP3
Inflammatory Response: CCL2 (MCP-1), FOS, IL1A, IL1R1, TLR7, TLR9.