分子量:46.5 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human TIM-3 (22-200), Fc Tag by the LAL method.

Buffer:50 mM tris, 100 mM glycine, pH7.5

生物活性:Measured by its binding ability in a functional ELISA. Immobilized human Galectin-9 at 500 ng/mL can bind Human TIM-3 (22-200), Fc Tag with an apparent KD <50 nM.

产品特性:Human TIM-3 (22-200), Fc Tag is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 46.5 kDa. The predicted N-terminus is Ser 22. As a result of glycosylation, the reducing (R) protein migrates as 55-66 kDa, and the non-reducing (NR) protein migrates as 110-130 kDa in SDS-PAGE .

产品背景:Hepatitis A virus cellular receptor 2 is also known as HAVCR2, FLJ14428, KIM3, TIM3, TIMD3, is a member of the TIM family of immune regulating molecules with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells and their associated cytokines are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. The 2 types of cells also cross-regulate the functions of the other. HAVCR2 is a Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice. HAVCR2 regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Dysregulation of the HAVCR2-galectin-9 pathway could underlie chronic autoimmune disease states in human, such as multiple sclerosis.