AIMP2,AIMP2抗体

产品编号HZ-2509R
英文名称AIMP2
中文名称AIMP2抗体
别 名Aminoacyl tRNA synthase complex-interacting multifunctional protein 2; Aimp2; AA407136; AA407225; Aimp2(p38); JTV 1 protein; Jtv1; MGC30261; MGC36346; MGC36522; MGC37389; ARS interacting multi functional protein 2; Multisynthetase complex auxiliary component p38; P38; tRNA SYNTHETASE COFACTOR p38; PRO0992; Protein JTV 1; JTV-1; JTV1; P38; PRO0992; AIMP2_HUMAN.
规格价格0.1ml 0.2ml 
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学 神经生物学 信号转导
抗体来源Rabbit
克隆类型Polyclonal
AIMP2,AIMP2抗体交叉反应Human, Mouse, Rat, Pig, Cow, Rabbit,
产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量36kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human AIMP2
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
AIMP2,AIMP2抗体PubMedPubMed
产品介绍background:
P38 is a probable core protein of the multisynthetase complex that serves as a template for the assembly of the supramolecular structure. Mediates ubiquitination of FUBP1 and its degradation by the proteasome. P38 is also a cofactor of multi-ARS complex and also a substrate of parkin in neuronal cell.

Function:
Required for assembly and stability of the minoacyl-tRNA synthase complex. Mediates ubiquitination and egradation of FUBP1, a transcriptional activator of MYC, leading o MYC down-regulation which is required for aveolar type II cell ifferentiation. Blocks MDM2-mediated ubiquitination and egradation of p53/TP53. Functions as a proapoptotic factor.

Subunit:
Component of the aminoacyl-tRNA synthase complex which is omprised of a bifunctional glutamyl-prolyl-tRNA synthase, the onospecific isoleucyl, leucyl, glutaminyl, methionyl, lysyl, rginyl and aspartyl-tRNA synthases, and three auxiliary proteins, EF1E1/p18, AIMP2/p38 and AIMP1/p43. Binds FUBP1 (via C-terminus). nteracts in both its unphosphorylated and phosphorylated forms ith p53/TP53 (via N-terminus) in the nucleus following UV rradiation. Interacts (via N-terminus) with KARS and PARK2/parkin via first RING-type domain).

Subcellular Location:
Cytoplasm, cytosol (By similarity). Nucleus By similarity). Note=Following DNA damage, dissociates from the minoacyl-tRNA synthase complex and translocates from the cytoplasm o the nucleus (By similarity).

Post-translational modifications:
Phosphorylated on serine residues in response to UV rradiation (By similarity).
Ubiquitinated by PARK2, leading to its degradation by the roteasome. Mutant PARK2 fails to ubiquitinate AIMP2 efficiently, llowing its accumulation which may contribute to neurodegeneration ssociated with Parkinson disease.

AIMP2,AIMP2抗体Similarity:
Contains 1 GST C-terminal domain.

Gene ID:
7965

Database links:
Entrez Gene: 7965 Human
Entrez Gene: 231872 Mouse
Entrez Gene: 288480 Rat
Omim: 600859 Human
SwissProt: Q13155 Human
SwissProt: Q8R010 Mouse
SwissProt: Q32PX2 Rat
Unigene: 301613 Human
Unigene: 728827 Human
Unigene: 200499 Mouse
Unigene: 162097 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-AIMP2 Polyclonal Antibody, Unconjugated(bs-2509R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining