分子量:54 kDa

纯度:>98% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human EpCAM, Fc Tag by the LAL method.

Buffer:50 mM tris, 100 mM glycine, pH7.5

生物活性:Measured by its binding ability in a functional ELISA. Immobilized recombinant human Cathepsin V at 10 μg/mL (100 μl/well ) can bind biotinylated EpCAM. The EC50 of biotinylated EpCAM is 80-100 ng/mL.

产品特性:Human EpCAM, Fc Tag is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 54 kDa. The predicted N-terminus is Gln 24. The reducing (R) protein migrates as 60-65 kDa in SDS-PAGE  due to glycosylation.

产品背景:EpCAM is also known as CO171A,; EGP; EGP40; GA7332; KSA; M4S; MIC18; MK1; TROP1; hEGP2, and is a pan-epithelial differentiation antigen that is expressed on almost all carcinomas as 17-1A(mAb) antigen. Its constitutional function is being elucidated. It is intricately linked with the Cadherin-Catenin pathway and hence the fundamental WNT pathway responsible for intracellular signaling and polarity. The epithelial cell adhesion molecule (Ep-CAM) is known to express in most epithelial malignancies and was reported as a tumor marker or a candidate of molecular targeting therapy.
Ep-CAM cross signaling with N-cadherin involves Pi3K, resulting in the abrogation of the cadherin adhesion complexes in epithelial cells was reported. And Epithelial cell adhesion molecule (Ep-CAM) recently received increased attention as a prognostic factor in breast cancer.