Description

Neurotrophin-3 (NT-3) is a new member of the nerve growth factor (NGF) gene family, and plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, neurotrophic tyrosine kinase receptor type 3 (Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest (NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.

ScienCells mouse Neurotrophin-3 ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse Neurotrophin-3-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The mouse-specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of mouse Neurotrophin-3 in the sample that is captured on the strips.

Product Use

This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.

Storage

Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles.

Shipping

Shipped on gel ice.

Reference

1. Ozcelik, T.; Rosenthal, A.; Francke, U. Chromosomal mapping of brain-derived neurotrophic factor and neurotrophin-3 genes in man and mouse. Genomics 10: 569-575, 1991.
2. Tessarollo, L.; Vogel, K. S.; Palko, M. E.; Reid, S. W.; Parada, L. F. Targeted mutation in the neurotrophin-3 gene results in loss of muscle sensory neurons. Proc. Nat. Acad. Sci. 91: 11844-11848, 1994.
3. Donovan, M. J.; Hahn, R.; Tessarollo, L.; Hempstead, B. L. Identification of an essential nonneuronal function of neurotrophin 3 in mammalian cardiac development. Nature Genet. 14: 210-213, 1996.
4. Kalcheim, C.; Carmeli, C.; Rosenthal, A. Neurotrophin 3 is a mitogen for cultured neural crest cells. Proc. Nat. Acad. Sci. 89: 1661-1665, 1992.
  

 

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