CD146,黑色素瘤细胞粘附分子CD146抗体

产品编号HZ-1618R
英文名称CD146
中文名称黑色素瘤细胞粘附分子CD146抗体
别 名A32 antigen; CD 146; CD146; CD146 antigen; Cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; MCAM; Melanoma adhesion molecule; Melanoma associated antigen A32; Melanoma associated antigen MUC18; Melanoma associated glycoprotein MUC18; Melanoma cell adhesion molecule; MelCAM; MUC 18; MUC18; S endo 1; S endo 1 endothelial associated antigen; Sendo 1 endothelial associated antigen; Sendo1.
说 明 书0.1ml 0.2ml
研究领域肿瘤 免疫学 细胞粘附分子 细胞表面分子
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Dog, Cow, Horse,
CD146,黑色素瘤细胞粘附分子CD146抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC= IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量71kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human CD146 (203-240aa)
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
CD146,黑色素瘤细胞粘附分子CD146抗体PubMedPubMed
产品介绍background:
MCAM (MUC18 antigen, CD146), a member of the immuoglobulin superfamily, is an intrinsic membrane glycoprotein of 110-120 kDa found on the surface of endothelial cells, bone marrow fibroblasts and various melanomas. MCAM (Melanoma adhesion molecule) has been used as a marker of tumor progression in human melanoma because expression in those tumors correlates strongly with poor prognosis and the development of metastic disease. In addition, a number of human T, B and myeloid leukemic cell lines seem to express MCAM. The close structural relationship with N-CAM and related molecules suggests that MCAM may be also a developmentally regulated cell adhesion.

Function:
Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Tissue Specificity:
Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.

CD146,黑色素瘤细胞粘附分子CD146抗体Similarity:
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 Ig-like V-type (immunoglobulin-like) domains.

Gene ID:
4162

Database links:
Entrez Gene: 4162 Human
Entrez Gene: 84004 Mouse
Entrez Gene: 78967 Rat
Omim: 155735 Human
SwissProt: P43121 Human
SwissProt: Q8R2Y2 Mouse
SwissProt: Q9EPF2 Rat
Unigene: 599039 Human
Unigene: 275003 Mouse
Unigene: 2694 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

CD146分子主要是选择性的在肿瘤血管内皮细胞中表达较高。