MSLN monoclonal antibody, clone MN-1
产品名称: MSLN monoclonal antibody, clone MN-1
英文名称: MSLN monoclonal antibody, clone MN-1
产品编号: MAB1993
产品价格: null
产品产地: 台湾
品牌商标: Abnova
更新时间: null
使用范围:
亚诺法生技股份有限公司(Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against partial recombinant MSLN.
- Immunogen:
- Recombinant protein corresponding to extracellular region of human MSLN.
- Host:
- Mouse
- Reactivity:
- Human
- Specificity:
- This antibody is directed against human mesothelin protein.
- Form:
- Liquid
- Storage Buffer:
- In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
- Recommend Usage:
- Western Blot (1:1000)
Immunohistochemistry (1:100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Mesothelin expression in human lung cancer.
Ho M, Bera TK, Willingham MC, Onda M, Hassan R, FitzGerald D, Pastan I.Clin Cancer Res. 2007 Mar 1;13(5):1571-5.
- 2.
- New monoclonal antibodies to mesothelin useful for immunohistochemistry, fluorescence-activated cell sorting, Western blotting, and ELISA.
Onda M, Willingham M, Nagata S, Bera TK, Beers R, Ho M, Hassan R, Kreitman RJ, Pastan I.Clin Cancer Res. 2005 Aug 15;11(16):5840-6.
- 3.
- Isolation and characterization of a monoclonal antibody, K1, reactive with ovarian cancers and normal mesothelium.
Chang K, Pastan I, Willingham MC.Int J Cancer. 1992 Feb 1;50(3):373-81.
- Applications
- Western Blot (Recombinant protein)
- Western blotting using MSLN monoclonal antibody, clone MN-1 (Cat # MAB1993) to detect MSLN at 100 ng (lane 1), 25 ng (lane 2), 6 ng (lane 3), 2 ng (lane 4) and 0.4 ng (lane 5).
Lane 6 contains 50 ng of CDC25-Fc.
Proteins were separated on 4-20% gradient gel by SDS-PAGE followed by transfer to PVDF membrane.
Primary antibody was used at 1 ug/mL followed by reaction with ALP goat anti-mouse IgG and BCIP/NBT substrate.
Reprinted with permission from Clin. Cancer Res. 11 (16) : 5840-6.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Immunohistochemistry using MSLN monoclonal antibody, clone MN-1 (Cat # MAB1993) to detect MSLN in PEFF (effective permeability) human tissue sections treated by antigen retrieval methods.
MSLN monoclonal antibody, clone MN-1 (Cat # MAB1993) were used to label these sections as follows : C, MAb MB; and D, MAb MN.
Reprinted with permission from Clin. Cancer Res. 11 (16) : 5840-6.
- Entrez GeneID:
- 10232
- Protein Accession#:
- NP_005814;Q13421
- Gene Name:
- MSLN
- Gene Alias:
- CAK1,MPF,SMR
- Gene Description:
- mesothelin
- Omim ID:
- 601051
- Gene Ontology:
- Hyperlink
- Gene Summary:
- An antibody that reacts with ovarian cancers and mesotheliomas was used to isolate a cell surface antigen named mesothelin. Although the function of mesothelin is unknown, it may play a role in cellular adhesion and is present on mesothelium, mesotheliomas, and ovarian cancers. This gene encodes a preproprotein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Alternative splicing results in multiple transcript variants. [provided by RefSeq
- Other Designations:
- megakaryocyte potentiating factor