phospho-cGKI (Thr515),磷酸化cGMP依赖性蛋白激酶1抗体

产品编号HZ-7368R
英文名称phospho-cGKI (Thr515)
中文名称磷酸化cGMP依赖性蛋白激酶1抗体
别 名phospho-PKG(Thr515); p-PKG(Thr515); cGKI alpha; CGKI; cGKI beta; cGMP-dependent protein kinase 1; KGPB; pkg; PRKG1; PRKG1B; PRKGR1A; PRKGR1B.
说 明 书0.1ml
产品类型磷酸化抗体
研究领域心血管 细胞生物 信号转导 激酶和磷酸酶
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat,
phospho-cGKI (Thr515),磷酸化cGMP依赖性蛋白激酶1抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量74kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human cGKI around the phosphorylation site of Thr515
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
phospho-cGKI (Thr515),磷酸化cGMP依赖性蛋白激酶1抗体PubMedPubMed
产品介绍background:
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. PKG plays an important stimulatory role in platelet activation.

Function:
Serine/threonine protein kinasethat acts as key mediator of the nitric oxide (NO)/cGMP signaling pathway. GMP binding activates PRKG1, which phosphorylates serines and threonines on many cellular proteins. Numerous protein targets for PRKG1 phosphorylation are implicated in modulating cellular calcium, but the contribution of each of these targets may vary substantially among cell types. Proteins that are phosphorylated by PRKG1 regulate platelet activation and adhesion, smooth muscle contraction, cardiac function, gene expression, feedback of the NO-signaling pathway, and other processes involved in several aspects of the CNS like axon guidance, hippocampal and cerebellar learning, circadian rhythm and nociception. Smoth muscle relaxation is mediated through lowering of intracellular free calcium, by desensitization of contractile proteins to calcium, and by decrease in the contractile state of smooth muscle or in platelet activation. Regulates intracellular calcium levels via several pathways: phosphorylates MRVI1/IRAG and inhibits IP3-induced Ca(2+) release from intracellular stores, phosphorylation of KCNMA1 (BKCa) channels decreases intracellular Ca(2+) levels, which leads to increased opening of this channel. PRKG1 phosphorylates the canonical transient receptor potential channel (TRPC) family which inactivates the associated inward calcium current. Another mode of action of NO/cGMP/PKGI signaling involves PKGI-mediated inactivation of the Ras homolog gene family member A (RhoA). Phosphorylation of RHOA by PRKG1 blocks the action of this protein in myriad processes: regulation of RHOA translocation; decreasing contraction; controlling vesicle trafficking, reduction of myosin light chain phosphorylation resulting in vasorelaxation. Activation of PRKG1 by NO signaling alters also gene expression in a number of tissues. In smooth muscle cells, increased cGMP and PRKG1 activity influence expression of smooth muscle-specific contractile proteins, levels of proteins in the NO/cGMP signaling pathway, down-regulation of the matrix proteins osteopontin and thrombospondin-1 to limit smooth muscle cell migration and phenotype. Regulates vasodilator-stimulated phosphoprotein (VASP) functions in platelets and smooth muscle.

Subunit:

Subcellular Location:
Cytoplasm. Colocalized with TRPC7 in the plasma membrane.

Tissue Specificity:
Primarily expressed in lung and placenta.

phospho-cGKI (Thr515),磷酸化cGMP依赖性蛋白激酶1抗体Post-translational modifications:
Autophosphorylation increases kinase activity. 65 kDa monomer is produced by proteolytic cleavage.

Similarity:
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
cGMP subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 cyclic nucleotide-binding domains.
Contains 1 protein kinase domain.

Gene ID:
5592

Database links:
Entrez Gene: 5592 Human
Omim: 176894 Human
SwissProt: Q13976 Human
Unigene: 407535 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.