GLS monoclonal antibody (M01), clone 5C4
产品名称： GLS monoclonal antibody (M01), clone 5C4
英文名称： GLS monoclonal antibody (M01), clone 5C4
产品编号： H00002744-M01
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant GLS.
- Immunogen:
- GLS (NP_055720, 580 a.a. ~ 669 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- EQRDYDSRTALHVAAAEGHVEVVKFLLEACKVNPFPKDRWNNTPMDEALHFGHHDVFKILQEYQVQYTPQGDSDNGKENQTVHKNLDGLL
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG2a Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.64 KDa) .
- MSDS:
Download
- Publication Reference
- 1.
- Mitochondrial localization and structure-based phosphate activation mechanism of Glutaminase C with implications for cancer metabolism.
Cassago A, Ferreira AP, Ferreira IM, Fornezari C, Gomes ER, Greene KS, Pereira HM, Garratt RC, Dias SM, Ambrosio AL.Proc Natl Acad Sci U S A. 2012 Jan 24;109(4):1092-7. Epub 2012 Jan 6.
- 2.
- Premature senescence of human endothelial cells induced by inhibition of glutaminase.
Unterluggauer H, Mazurek S, Lener B, Hutter E, Eigenbrodt E, Zwerschke W, Jansen-Durr P.Biogerontology. 2008 Aug;9(4):247-59. Epub 2008 Mar 4.
- Applications
- Western Blot (Recombinant protein)
- Protocol Download
- Immunofluorescence
enlarge this image- Immunofluorescence of monoclonal antibody to GLS on HeLa cell. [antibody concentration 10 ug/ml]
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged GLS is approximately 0.3ng/ml as a capture antibody.
- Protocol Download
- Entrez GeneID:
- 2744
- GeneBank Accession#:
- NM_014905
- Protein Accession#:
- NP_055720
- Gene Name:
- GLS
- Gene Alias:
- AAD20,DKFZp686O15119,FLJ10358,GLS1,KIAA0838
- Gene Description:
- glutaminase
- Omim ID:
- 138280
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Sahai (1983) [PubMed 6825316] demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter (Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.[supplied by OMIM
- Other Designations:
- K-glutaminase,L-glutamine amidohydrolase,glutaminase C,glutaminase, phosphate-activated