分子量:33 kDa

纯度:>92% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Biotinylated Mouse Fc gamma RI by the LAL method.

Buffer:PBS, pH7.4

生物活性:The bioactivity is measured by its binding ability to mouse IgG2a in a SPR assay.
Immobilized Biotinylated Mouse Fc gamma RI / CD64 Protein (Cat# CD4-M52E8) can bind mouse IgG2a with affinity constant of around 6.0 nM.
Measured by its binding ability in a functional ELISA.Immobilized Yervoy Ipilimumab (Human IgG1) at 10 μg/mL (100 μL/well) can bind Biotinylated Mouse Fc gamma RI (Cat# CD4-M52E8 ) with a linear range of  0.03-0.5 μg/mL.


产品特性:This protein carries a 15 AA fragment at the C-terminus, followed by a polyhistidine tag. There is one single lysine residue within the 15 AA fragment that is enzymatically labeled with biotin in vivo. The protein has a calculated MW of 33 kDa. The predicted N-terminus is Glu 25. The reducing (R) protein migrates as 45-60 kDa in SDS-PAGE due to glycosylation.

产品背景:Receptors that recognize the Fc portion of IgG are divided into three groups designated Fc gamma RI, RII, and RIII, also known respectively as CD64, CD32, and CD16. Fc gamma RI binds IgG with high affinity and functions during early immune responses. Fc gamma RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses.
High affinity immunoglobulin gamma Fc receptor I is also known as FCGR1A, FCG1, FCGR1, CD64 and IGFR1, is a type of integral membrane glycoprotein that binds monomeric IgG-type antibodies with high affinity, which belongs to the immunoglobulin superfamily or FCGR1 family. FCGR1A / CD64 contains 3 Ig-like C2-type (immunoglobulin-like) domains. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells.